EDTA and cell lysis

Barbara MacGregor via methods%40net.bio.net (by bmacgreg from unc.edu)
Wed Oct 31 12:23:41 EST 2007


At least some bacteria can be lysed with EDTA treatment alone, which  
permeabilizes the membrane - can't remember the details of the  
mechanism off the top of my head...


On Oct 31, 2007, at 1:05 PM, methods-request from oat.bio.indiana.edu wrote:

> Dear friends, I got protocol from pharmacia for Gram negative  
> bacterial cell
> lysis with lysozyme and EDTA. I have gone through several  
> literature where
> they have used EDTA with lysozyme mediated cell lysis of Gram negative
> bacteria but not in case of Gram positive bacteria.
> Can anybody tell me why EDTA is used specially for Gram negative  
> bacteria,
> the exact mechanism behind it
> Regards.
> -- 
> Chiranjit Chowdhury
> Department of Biotechnology
> Indian Institute of Technology, Kharagpur
> Kharagpur, West Bengal, India
> Pin: 721302

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