kDa limit E. coli expression

Soenke Behrends via methods%40net.bio.net (by s.behrends from tu-bs.de)
Mon Sep 10 08:21:24 EST 2007


Dear All,
is there a size limit for soluble expression of proteins in E. coli? 
Have codon optimized artificial genes pushed the limits? Is it 
reasonable to assume that it is difficult above 50 kDa and impossible 
above 100 kDa to get soluble expression in E. coli?
Thanks for your kind help!
Soenke



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