need ~100 bp fragments from Saliva DNA

chovek69 via methods%40net.bio.net (by ivanoov from gmail.com)
Sat Sep 15 01:16:03 EST 2007


On Sep 14, 11:47 pm, Keyz Tolope <keyztol... from yahoo.com> wrote:
> Hello,
> I need to cleave DNA purified from saliva to about 100
> bp fragments.  I have tried sonication, but it gives
> broad results from ~200-600 bp.  I do not know the
> sequence, although the dna is around 12-14kb.  What
> would the most efficient way to do this be?  Perhaps a
> combination of sonication with some sort of enzymatic
> digestion?
>
> Thanks!
>
>       ___________________________________________________________________________­_________
> Check out the hottest 2008 models today at Yahoo! Autos.http://autos.yahoo.com/new_cars.html

You can try to autoclave it for 30min to 1h. From my experience you
cannot amplify anything above 100bp after that.



More information about the Methods mailing list