need ~100 bp fragments from Saliva DNA

Ozan Aygun via methods%40net.bio.net (by metugenetics from yahoo.com)
Sat Sep 15 13:39:11 EST 2007


Hi,

I suggest you to try partial digestion with
Micrococcal nuclease or Benzonase. 

Treatment with Benzonase decreases viscosity very
quickly when incubated >20 C and has a broad range
buffer compatibility. You can check the size of the
fragments in a time course titration experiment with
benzonase incubation & concentration and optimize your
desired DNA size.

Hope this may be any help, good luck!

Ozan


       
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