beta-gal senescence staining of mouse tissue

AS via methods%40net.bio.net (by Antonio.Sarikas from gmail.com)
Wed Sep 19 13:24:01 EST 2007


Hi,

I am planning to do immunohistochemistry with embryonic / adult KO
mouse tissue to look for signs of senescence  by X-Gal staining (kit
from Cell Signaling Tech). Do you think these tissue should be
formalin/paraffin embeded or snap frozen? Also can these tissues be
collected for a couple of weeks and then stained?

Thanks for your comments
Tony



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