in-vitro systems

[corona]

> Something elese comes into my mind: Do you have ribosome biding site
> (Kozak consensus element, reads gccaccaccATGg or similar) in your
> construct? Not sure if applicable: Do you get expression, when you
> transfect the construct in cells?

Yes, there is a ribosome binding site (luciferase ATG translation start 
site). Although the flanking sequence surrounding the ATG may not be the 
optimal Kozak consensus sequence.

I have not tried in-vivo transformation yet, but considering my in-vitro 
results I would expect the same results.