(by customer from newsnet.com)
Tue Sep 18 19:45:40 EST 2007
> Something elese comes into my mind: Do you have ribosome biding site
> (Kozak consensus element, reads gccaccaccATGg or similar) in your
> construct? Not sure if applicable: Do you get expression, when you
> transfect the construct in cells?
Yes, there is a ribosome binding site (luciferase ATG translation start
site). Although the flanking sequence surrounding the ATG may not be the
optimal Kozak consensus sequence.
I have not tried in-vivo transformation yet, but considering my in-vitro
results I would expect the same results.
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