Heat-stable T4 lysozyme

Barbara MacGregor via methods%40net.bio.net (by bmacgreg from unc.edu)
Thu Sep 20 16:10:27 EST 2007


Actually there are heat-stable mutants of T4 lysozyme, but I have no  
idea if they're commercially available. Here's an old reference:  
Wetzel et al. (1988) PNAS 85:401-405. I guess that would show you how  
to make your own!


>
> From: "Virash Gupta" <virashkgupta from gmail.com>
> Date: September 19, 2007 12:14:13 AM EDT
> To: methods from oat.bio.indiana.edu
> Subject: T4 lysozyme
>
>
> T4 lysozyme can not be heat stable. Look for enzyme produced by
> thermophilic organism or recombinant E.coli with thermophilic lysozyme
> gene from thermophilic organism like Thermus aquaticus.
>
> On 9/18/07, methods-request from oat.bio.indiana.edu
> <methods-request from oat.bio.indiana.edu> wrote:
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>>   1. Re: T4lysozyme (DK)
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>> --------------------------------------------------------------------- 
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>> Message: 1
>> Date: Mon, 17 Sep 2007 21:54:39 GMT
>> From: dk from no.email.thankstospam.net (DK)
>> Subject: Re: T4lysozyme
>> To: methods from net.bio.net
>> Message-ID: <AICHi.850$3C.659 from newsfe05.lga>
>>
>> Fathi Hassan <fhassan69 from yahoo.com> wrote:
>>>
>>> Dear all,
>>> I'm looking for heat stable T4-lysozyme, can any one kindly has=
>>> this gene or tell me from whom I can get it.
>>> Thank you very much in Advance
>>
>> Someone correct me if I am wrong but I don't think
>> T4 lysozyme is heat stable.
>>



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