pBR322 plasmids

Michael Sullivan via methods%40net.bio.net (by mlsulliv from wisc.edu)
Mon Sep 24 09:09:03 EST 2007


On Sep 23, 2007, at 6:08 PM, peter wrote:

> On Sep 11, 10:37 pm, Aawara Chowdhury <aaw... from FEMA-trailer.org> wrote:
>> No.  You'll have to put different origins on the two plasmids.
>>
>> In <mailman.899.1189533811.11350.meth... from net.bio.net>,
>>  Sylvie Chauvaux <chauv... from pasteur.fr> wrote:
>>
>>> Dear all,
>>
>>> Can 2 plasmids carrying the same pBR322 origin of replication and 2
>>> different antibiotic resistance cassettes co-reside in the same
>>> strain of E. coli ?`
>>
>>> Thank you for your help,
>>
>>> Sylvie
>>
>> --
>> Email: echo 36434455860060025978157675027927670979097959886449930P  
>> | dc
>
> Yes, They perfectly can exist if you put the two antibiotics
>

I'd worry if one of the markers were Amp, since that antibiotic is  
depleted from media pretty quickly.

Mike
---
Michael L. Sullivan
Plant Research Molecular Geneticist
US Dairy Forage Research Center
ARS-USDA
1925 Linden Drive West
Madison, WI 53706
(608) 890-0046 (Phone)
(608) 890-0076 (FAX)



More information about the Methods mailing list