minimum radioactive probe size

[Ed Siefker]

I'm considering doing a lambda plaque hybridization with a very small 
probe.   The unique sequence I'm looking for is 43bp long.  I'm lucky to 
have that much really.  We do our colony hybridizations exactly like we 
would do a southern.

So has anyone done a hybridization (plaque or southern) with a tiny 
probe like this? I imagine it might be hard to get a high enough 
specific activity, but I think it's worth a shot.