calculate Kcat from Vmax

pp_fon via (by pesutcha from
Thu Sep 27 05:09:44 EST 2007

Dear all
  i wonder how to calculate a kcat from Vmax cause i get the Vmax value by
use a Michalis-Menten plot and the Unit of Vmax value is
umol/min/mg(enzyme). and i use 0.0004 mg of enzyme per reaction and the MW
of enzyme is 64 kDa. How can i calculate the Vmax value
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