Coated plates for fluorescence imaging of baculovirus-infected
(by jma from ddt.biochem.umn.edu)
Tue Apr 1 18:37:06 EST 2008
Which coating is best to keep baculovirus-infected insect cells adhered to microscope plates: poly-lysine, integrin, laminin, etc? Any recommendations on plate type and vendor availability?
I have a major problem doing FRET microscopy on Sf21 insect cells infected with recombinant AcNPV baculoviruses: following infection, the Sf21 cells detach from the glass microscope plate and roll around, especially when I change filter cubes (donor, acceptor, FRET dichroics) during photobleaching experiments.
I am expressing ER membrane proteins tagged with cyan and yellow fluorescent proteins (CFP, YFP).
J. Michael Autry, PhD
Research Associate, University of Minnesota
Minnesota Muscle Laboratory, David D. Thomas, PI
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