Why use K+ and NH4+ in Taq buffer?
(by stxsz1 from nottingham.ac.uk)
Wed Apr 2 17:19:58 EST 2008
Can someone explain to me what is the role of K+ and NH4+ in the PCR buffer?
I noticed most of the suppliers are using the NH4 buffer. Only NEB sticks to the original KCl buffer.
Bioline 1x Taq buffer: 16mM (NH4)2SO4, 67mM Tris
NEB 1x Taq standard buffer: 50mM KCl, 10 mM Tris
and why the Bioline use such a high concentration of Tris?
Thanks in advance
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