Real time PCR. Funky fluorescence?

[Matti Savoila]

Hello everyone!

I am doing some real time PCR with the LightCycler 1.5 instrument, but
I'm getting weird results. The fluorescence rises, like it should, but
suddenly in one cycle it plummets down. Examples:
http://www.mv.helsinki.fi/savoila/d_kuvat/Nayte_4_edit.jpg
http://www.mv.helsinki.fi/savoila/d_kuvat/Nayte_6_edit.jpg

Have you seen behaviour like this, and more importantly, have you seen
a solution to problems like this? Number 10 in both samples has been
amplified normally, which is strange. If somebody has some ideas, I'd
be thankful.

Best regards
Matti Savoila