Old school 2D gel electrophoresis (Arne K Christensen)

[Wenta Nikola]

Hi Arne,
You could at first run a "traditional" 1D-Gel (IEF, in example), then
you cut out the lanes and place them on top of a stacking gel (with
separating gel below) and run it as a "multilane" sample; to be sure
that each lane run properly in the 1st dimension, you could load your
sample between two marker lanes. Biorad offers "two lane combs"
(165-3356 Mini-PROTEAN Comb, prep/2-D well, 0.75 mm) with one pocket for
the marker and one broad "lane" for the gel slice (horizontal). The
remaining space around the cut slice, you can fill up with sample
buffer.
Cheers,
Niko

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