Page gel

Jayakumar, R via methods%40net.bio.net (by R.Jayakumar from roswellpark.org)
Tue Apr 29 12:44:13 EST 2008


I think he meant VOLTS or Amp and not WATTS.  If you run it at 4 Watts,
that must be a very low volts.
  Jay
 

-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu
[mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of yan sun
Sent: Sunday, April 27, 2008 11:39 PM
To: Haviland, David L
Cc: Methods from magpie.bio.indiana.edu
Subject: Re: Page gel

 run 20% gel 4w around 10 hours, but the ladder is diffused, I mean the
band is really wide"( did it happen to you?
if you run under very low watage, it is easy to diffuse.



On 4/27/08, Haviland, David L <David.L.Haviland from uth.tmc.edu> wrote:
>
>
> The reasons you cite is exactly why I always ran my gels on constant
amps
> rather than volts.   For a 10% SDS-PAGE I could start it at 5 pm and
by 9 am
> the next morning it was about 3 cm from the bottom.   Even during the
day I
> never ran them above 25 mA without some form of cooling else they'd
"smile"
> and make interpretation a bit problematic.    In my hands, slower was
always
> better.
>
> David
>
> -----Original Message-----
> From: methods-bounces from oat.bio.indiana.edu on behalf of yan sun
> Sent: Sat 4/26/2008 6:38 PM
> To: Methods from magpie.bio.indiana.edu
> Subject: Page gel
>
> Dear all,
> I got a question about PAGE electrophoresis gel to confirm.
> When we mention that proper volts condition for running a gel is 
> around 20V/cm.
> For eample, my gel is 20 cm, which means that proper Volts is ~400V? 
> Am I right?
> If the volts is toooo high, the gel might toooo hot to get good 
> resolution, right?
> Thx a lot!!!!
>
> Frances
> --
> Y. F.S.
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
>


--
Y. F.S.
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