Page gel

Jayakumar, R via (by R.Jayakumar from
Tue Apr 29 12:44:13 EST 2008

I think he meant VOLTS or Amp and not WATTS.  If you run it at 4 Watts,
that must be a very low volts.

-----Original Message-----
From: methods-bounces from
[mailto:methods-bounces from] On Behalf Of yan sun
Sent: Sunday, April 27, 2008 11:39 PM
To: Haviland, David L
Cc: Methods from
Subject: Re: Page gel

 run 20% gel 4w around 10 hours, but the ladder is diffused, I mean the
band is really wide"( did it happen to you?
if you run under very low watage, it is easy to diffuse.

On 4/27/08, Haviland, David L <David.L.Haviland from> wrote:
> The reasons you cite is exactly why I always ran my gels on constant
> rather than volts.   For a 10% SDS-PAGE I could start it at 5 pm and
by 9 am
> the next morning it was about 3 cm from the bottom.   Even during the
day I
> never ran them above 25 mA without some form of cooling else they'd
> and make interpretation a bit problematic.    In my hands, slower was
> better.
> David
> -----Original Message-----
> From: methods-bounces from on behalf of yan sun
> Sent: Sat 4/26/2008 6:38 PM
> To: Methods from
> Subject: Page gel
> Dear all,
> I got a question about PAGE electrophoresis gel to confirm.
> When we mention that proper volts condition for running a gel is 
> around 20V/cm.
> For eample, my gel is 20 cm, which means that proper Volts is ~400V? 
> Am I right?
> If the volts is toooo high, the gel might toooo hot to get good 
> resolution, right?
> Thx a lot!!!!
> Frances
> --
> Y. F.S.
> _______________________________________________
> Methods mailing list
> Methods from

Y. F.S.
Methods mailing list
Methods from

This email message may contain legally privileged and/or confidential information.  If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited.  If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.

More information about the Methods mailing list