Page gel

[Gilbride, Kevin]

20% is high for acrylamide. Have you considered a tricine gel? If you
absolutely need sds page 4c @ 18mA overnight should work fine. Also, if
you are using a premixed concentrate for your running buffer the pH can
be affected by your water source.
K
 -----Original Message-----
From: methods-bounces from oat.bio.indiana.edu
[mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of yan sun
Sent: Sunday, April 27, 2008 11:39 PM
To: Haviland, David L
Cc: Methods from magpie.bio.indiana.edu
Subject: Re: Page gel


 run 20% gel 4w around 10 hours, but the ladder is diffused, I mean the
band is really wide"(
did it happen to you?
if you run under very low watage, it is easy to diffuse.



On 4/27/08, Haviland, David L <David.L.Haviland from uth.tmc.edu> wrote:
>
>
> The reasons you cite is exactly why I always ran my gels on constant
amps
> rather than volts.   For a 10% SDS-PAGE I could start it at 5 pm and
by 9 am
> the next morning it was about 3 cm from the bottom.   Even during the
day I
> never ran them above 25 mA without some form of cooling else they'd
"smile"
> and make interpretation a bit problematic.    In my hands, slower was
always
> better.
>
> David
>
> -----Original Message-----
> From: methods-bounces from oat.bio.indiana.edu on behalf of yan sun
> Sent: Sat 4/26/2008 6:38 PM
> To: Methods from magpie.bio.indiana.edu
> Subject: Page gel
>
> Dear all,
> I got a question about PAGE electrophoresis gel to confirm.
> When we mention that proper volts condition for running a gel is
around
> 20V/cm.
> For eample, my gel is 20 cm, which means that proper Volts is ~400V?
Am I
> right?
> If the volts is toooo high, the gel might toooo hot to get good
> resolution,
> right?
> Thx a lot!!!!
>
> Frances
> --
> Y. F.S.
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
>


-- 
Y. F.S.
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