How to make polymerase stop in sequence?

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Mon Aug 4 05:25:59 EST 2008


Dear Experts,

is there a way how one can make the DNA polymerase (ordinary Taq
should do, no proofreading activity is desired) stop within a sequence
introduced by a synthetic primer? I thought of something like several
sugar building blocks without any base attached or a modified base
which is so bulky that the pol must stop and slip off.
The modified position does not need to align to the parent DNA strand
as it may be placed in a loop. It just should be able to be
synthesized with standard phosphoramidite technology.

Many thanks for sharing your thoughts,

Wo


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