HELP! protein-protein cross-linking (Dr Engelbert Buxbaum)

Nikola Wenta via methods%40net.bio.net (by Nikola.Wenta from nottingham.ac.uk)
Fri Aug 8 12:10:49 EST 2008


Hi again!
I think that Dr. Buxbaum is absolutely right. Analytical Ultracentrifugation is definitely the method of choice: you can prove assoziation, get Kds, stoichiometries, even shapes ;-)
You wrote that you have solved a structure; X-ray, right? Problem: solid crystal; what you try now is interactions in solution... You should also think about Static Light Scattering; often, SLS and AUC are used in parallel because they show similar things by different methods.
Cheers,
Niko

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