David-Paul Minde via (by davidminde from
Tue Aug 12 07:31:30 EST 2008

... (hope you have no longer any problems with mutagenesis as in a post some
6 years back but ..) I strongly prefer Phusion (=highest fidelity
commercially available polymerase) PCR for mutagenesis. Piece of cake easy
for up to ~20 kb plasmids :) ...



David Minde MSc (TUM)

Cellular Protein Chemistry Room 707
Department of Chemistry
Faculty of Science
Utrecht University
Padualaan 8
NL-3584 CH Utrecht
The Netherlands

office phone +31 30 253 4105

private address:
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3572 TW Utrecht

I never think of the future. It comes soon enough.
Albert Einstein
Res severa verum gaudium. (~true delight is a severe issue)

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