mutagenesis

[David-Paul Minde]

... (hope you have no longer any problems with mutagenesis as in a post some
6 years back but ..) I strongly prefer Phusion (=highest fidelity
commercially available polymerase) PCR for mutagenesis. Piece of cake easy
for up to ~20 kb plasmids :) ...

best,

David

-- 
David Minde MSc (TUM)

Cellular Protein Chemistry Room 707
Department of Chemistry
Faculty of Science
Utrecht University
Krytgebouw
Padualaan 8
NL-3584 CH Utrecht
The Netherlands

office phone +31 30 253 4105

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Albert Einstein
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Seneca