charge on protein

WS via (by novalidaddress from
Mon Aug 18 17:52:55 EST 2008

Dear Rashmi,

you could use the "pI Tool" from Expasy as long as you know your
sequence. Of course, when your protein is
modified the results need corrections. A better way might be a 2D Gel
(or at least 1D-isoelectric focusing) where you may determine the
*real* state of your buddy.

Best regards,


On 18 Aug., 14:32, "Rashmi Srivastava" <rashmi1... from>
> Hello Experts,
> What simple methods can I use to determine the overall charge on a membrane
> protein??
> Rashmi

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