charge on protein

[WS]

Dear Rashmi,

you could use the "pI Tool" from Expasy as long as you know your
sequence. http://www.expasy.org/ Of course, when your protein is
modified the results need corrections. A better way might be a 2D Gel
(or at least 1D-isoelectric focusing) where you may determine the
*real* state of your buddy.

Best regards,

Wo



On 18 Aug., 14:32, "Rashmi Srivastava" <rashmi1... from googlemail.com>
wrote:
> Hello Experts,
>
> What simple methods can I use to determine the overall charge on a membrane
> protein??
>
> Rashmi