Query about Maximum PCR product size for pGEM-T
(by ddilernia from fmed.uba.ar)
Wed Aug 20 11:01:10 EST 2008
Dear Tony Schountz,
My name is Dario and I am from Argentina. I am sending you this mail because
I am looking for information regarding the maximum PCR fragment size that
can be cloned into pGEM-T Easy vector and I found on the internet that you
have asked the same question in a web forum although I didn´t find whether
somebody answered you
Did you find this information? It would be very
helpful for me. I see that your question was posted in the year 2000 so may
be you already have experience in this field. Did you have success cloning
large fragments with pGEM-T?
Lic. Dario A. Dilernia
Centro Nacional de Referencia para el SIDA
Departamento de Microbiología, Facultad de Medicina - UBA
Paraguay 2155 Piso 11º - C1121ABG
Buenos Aires, ARGENTINA
Tel: 54 11 4508-3671/3689 int: 132
Fax: 54 11 4508-3705
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