Methods Digest, Vol 39, Issue 14

sankar jagarlamudi via methods%40net.bio.net (by biotech2020 from gmail.com)
Fri Aug 22 09:50:17 EST 2008


Respected Sir/ Madam,


             i register for this subscription and i dont know how to operat=
e
this for my correspondence. Please let me know how to operate this journal.

Thanks

On Thu, Aug 21, 2008 at 10:36 PM, <methods-request from oat.bio.indiana.edu>wrot=
e:

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>   1. Query about Maximum PCR product size for pGEM-T (Dario Dilernia)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 20 Aug 2008 13:01:10 -0300
> From: "Dario Dilernia" <ddilernia from fmed.uba.ar>
> Subject: Query about Maximum PCR product size for pGEM-T
> To: <methods from magpie.bio.indiana.edu>
> Message-ID: <000b01c902dd$f687e960$e397bc20$@uba.ar>
> Content-Type: text/plain;       charset=3D"iso-8859-1"
>
> Dear Tony Schountz,
>
>
>
> My name is Dario and I am from Argentina. I am sending you this mail
> because
> I am looking for information regarding the maximum PCR fragment size that
> can be cloned into pGEM-T Easy vector and I found on the internet that yo=
u
> have asked the same question in a web forum although I didn=B4t find whet=
her
> somebody answered you=85 Did you find this information? It would be very
> helpful for me. I see that your question was posted in the year 2000 so m=
ay
> be you already have experience in this field. Did you have success clonin=
g
> large fragments with pGEM-T?
>
>
>
> Best regards,
>
>
>
> Dario
>
>
>
>
>
>
>
>
>
> Lic. Dario A. Dilernia
>
>
>
> Centro Nacional de Referencia para el SIDA
>
> Departamento de Microbiolog=EDa, Facultad de Medicina - UBA
>
> Paraguay 2155 Piso 11=BA - C1121ABG
>
> Buenos Aires, ARGENTINA
>
> Tel: 54 11 4508-3671/3689 int: 132
>
> Fax: 54 11 4508-3705
>
>
>
>
>
>
> --
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> End of Methods Digest, Vol 39, Issue 14
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