Counterstain for AEC AND BCIP/NBT

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Wed Aug 27 14:10:34 EST 2008


Am 27.08.2008, 04:30 Uhr, schrieb Bluescript <shauke from uni-bremen.de>:

> Hi,
> I want to do immunohistochemistry detecting two targets at the same
> time. For various reasons I am going to use AEC as substrate for HRP
> and BCIP/NBT as AP substrate. Now I am looking for a good
> counterstain. Because of the AEC the counterstain has to work with an
> aqueous embedding medium.
> Has anybody a good idea? Thank you very much for your help in advance,

Intercalating DNA stains are commonly used to mark nuclei: bisbenzimide,  
DAPI, Propidium iodide and a host of proprietary dyes (check the Molecular  
Probes catalogue).


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