protocol for liposome preparation

jh via methods%40net.bio.net (by biocjh from gmail.com)
Wed Dec 3 09:18:02 EST 2008


Excuse me, may you explain more detail for "PC".
Whether the liposome is prepared for cell transfection experiment?

Jianhao

2008/11/30 WS <novalidaddress from nurfuerspam.de>:
> Hi Michael,
>
> you might try this: dissolve PC in EtOH and inject into 20 to 50 fold
> volume of buffer with an insulin syringe (or the narrowest needle you
> can get) while vortexing. If the EtOH might cause problems downstream,
> dialyze against buffer.
>
> Worked well for PC/Ceramide vesicles for me.
>
> best regards,
>
> Wo
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