protocol for liposome preparation

jh via methods%40net.bio.net (by biocjh from gmail.com)
Thu Dec 4 21:26:11 EST 2008


Thanks.
I wonder whether anyone prepare liposome by self for cell transfection
experiment?Cause ,I thought, this may help for someone who do not have
much fund.

rgds!

jianhao

2008/12/4, WS <novalidaddress from nurfuerspam.de>:
> On 3 Dez., 15:18, jh <bio... from gmail.com> wrote:
> > Excuse me, may you explain more detail for "PC".
> > Whether the liposome is prepared for cell transfection experiment?
> >
> > Jianhao
> >
> > 2008/11/30 WS <novalidaddr... from nurfuerspam.de>:
> >
> > > Hi Michael,
> Hi,
>
> PC means phosphatidylcholine. I haven't used them for transfection but
> to transfer a fluorescently labeled ceramid into fibroblasts.
>
> Regards,
>
> Wo
>
>
> > > you might try this: dissolve PC in EtOH and inject into 20 to 50 fold
> > > volume of buffer with an insulin syringe (or the narrowest needle you
> > > can get) while vortexing. If the EtOH might cause problems downstream,
> > > dialyze against buffer.
> >
> > > Worked well for PC/Ceramide vesicles for me.
> >
> > > best regards,
> >
> > > Wo
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