protocol for liposome preparation

jh via methods%40net.bio.net (by biocjh from gmail.com)
Sat Dec 6 05:10:38 EST 2008


thanks
Do you have the protocol in detail?
Or Would you like to recommendate one?

rgds!

Jh

2008/12/5, DK <dk from no.email.thankstospam.net>:
> In article <mailman.964.1228450225.29717.methods from net.bio.net>, jh <biocjh from gmail.com> wrote:
> >Thanks.
> >I wonder whether anyone prepare liposome by self for cell transfection
> >experiment?Cause ,I thought, this may help for someone who do not have
> >much fund.
>
> People who don't have funds to afford various lipofection reagents
> on the market would probably do well by carefully optimizing
> polyethyleneimine-mediated transfection. Lots of reports that suggest
> that PEI gives transfection efficiencies comparable to many cationic
> lipids.
>
> DK
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