PAGE gel sticked on both plates

jh via methods%40net.bio.net (by biocjh from gmail.com)
Mon Dec 8 02:50:11 EST 2008


how about trying to soak the gel and plates in buffer for seperation

2008/12/8 Nicosnake <chamosilencio from yahoo.com>:
>
> HI everyone, i've been having this problem whit my acrylamide gels: after
> acrylamide is polymerized between the two glass plates it's really hard to
> separate both plates and after a long effort i can take them appart only to
> realize that the gel has sticked to BOTH plates, the gel is actually cut in
> half. I treat surfaces of both plates, one with bind silane (so the
> acrylamide sticks to this one) and the other with Sigmacote to prevent the
> gel from sticking. But still the gel sticks to both surfaces and after i
> separate the glases it's useless. Has anyonehad this kind of trouble?? ANy
> suggestion is accepted.
> --
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