Co-IP problems

[Fulvio Celsi]

Dear All
I'm looking for  suggestions on CoImmunoPrecipitation.... The protein 
I'm interested in CoIP has a MW of 75Kd, so any time I made the 
experiment, on the CoIP lane I saw a smear from the Igg (around 60kd if 
I know well). How can I reduce this effect? I cannot buy another Ab 
(from a different species) thing that I suppose would help...can I 
cross-link ab to the protein-A beads? if yes...how??
Thanks a lot in advance
Fulvio

-- 
Fulvio Celsi
BsC,PhD
Dept. of Experimental and Diagnostic Medicine
Section of General Pathology
University of Ferrara
Via  Borsari 46
44100 Ferrara
Italy
Mobile: +393286489131

http://salvarelaricerca.blogspot.com/