Co-IP problems

[Fulvio Celsi]

Allison ha scritto:
> Fulvio Celsi wrote:
>> Dear All
>> I'm looking for  suggestions on CoImmunoPrecipitation.... The protein 
>> I'm interested in CoIP has a MW of 75Kd, so any time I made the 
>> experiment, on the CoIP lane I saw a smear from the Igg (around 60kd 
>> if I know well). How can I reduce this effect? I cannot buy another 
>> Ab (from a different species) thing that I suppose would help...can I 
>> cross-link ab to the protein-A beads? if yes...how??
>> Thanks a lot in advance
>> Fulvio
>>
>
> Could you run a non-reducing gel?  Then the IgG would run at 150K vs 
> 75K for your protein (assuming the latter is not subunits as well).
> Allison
ehm..unfortunatly not...the whole protein is bigger (tetramer)...but 
good idea...
thanks!
Fulvio

> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>

-- 
Fulvio Celsi
BsC,PhD
Dept. of Experimental and Diagnostic Medicine
Section of General Pathology
University of Ferrara
Via  Borsari 46
44100 Ferrara
Italy
Mobile: +393286489131

http://salvarelaricerca.blogspot.com/