Dr. Hiranya S. Roychowdhury
(by hroychow from nmsu.edu)
Fri Dec 19 12:29:33 EST 2008
I don't believe you can expect a solution of PP1 in PBS. It will form a
colloidal suspension. A colloidal suspension will also elicit immune
response. Remember that the peritonial fluid is also polar and the PP1
will come out of solution there too. DMSO, however, does the rat very
little damage in microliter quantities intra-peritonially. I believe the
PBS is used to minimize any shock. PP1 is soluble also in ethanol, and it
may be a better alternative solvent for the injection. Just a thought!
> To whom it may concern,
> I am from U of Toronto and have recently joined to your group. Had some
> problems in dissolving a compound and was wondering if I can get any
> suggestion from the expert people.
> I have to do i.p. injections to the rats and the compound of interest is
> kinase inhibitor PP1.
> http://www.biomol.com/SiteData/docs/productdata/ei275.pdf. As I got the
> information from the literature, it has been mentioned in several papers
> that this material is dissolved in DMSO and further diluted in the PBS
> to the injection to rats.
> In the data sheet of the company it is also mentioned that this material
> should be dissolved in DMSO (25 mg/ml). Each vial had 5mg in it, I
> initially added 200ul of DMSO to that and completely dissolve it by
> pipetting up and down, but when I added PBS (as indicated in the papers),
> all the compound was precipitated and I got just a colloidal solution. The
> company did not have any suggestion, as they told that it is hydrophobic
> material. I don't know how the papers have done that. On the other hand I
> have to dilute it in PBS because injecting DMSO to the rats has other
> effects which can interfere with my experimental plan. I should mention
> heating the compound up to 37C for several hours and its repetitive
> agitation did not help this compound go to the solution.
> I would appreciate if anybody can give suggestion in this regard and
> urgently waiting for the nswer,
> Methods mailing list
> Methods from net.bio.net
Hiranya S. Roychowdhury, Ph.D.
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003
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