methylcellulose colony question
(by SBrown from ccia.unsw.edu.au)
Fri Dec 19 12:33:48 EST 2008
I have been growing Nalm-6 colonies in methocult for about a year now, the ratio of media is 1.6ml methocult, 1.2ml IMDM and 1.2ml FCS with or without drug depending on what i am doing.
Has anyone had any experience with maintaining the methocult for at least 3 weeks, frequently my methocult looks as though is has dessicated, i have water in the incubator and i also plate 2 wells with PBS to ensure humidity...but...it still happens, any ideas or suggestions?
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