need plasmid map or sequence - PT81-Luc
(by aawara from pontiff-playground.org)
Tue Dec 30 10:24:00 EST 2008
In <IDj6l.46555$Uk3.15319 from newsfe10.iad>,
DK <dk from no.email.thankstospam.net> wrote:
> In article <e1388331-429c-4d0f-a43c-c3f27e4ddb07 from s37g2000vbp.googlegroups.com>, egrasso <estebangrasso from gmail.com> wrote:
>>Hi, I need the plasmid map or sequence (i prefer the sequence) of the
>>plasmid pT81-Luc. It is a Luciferase reporter plasmid with a minimal
>>thymidine kinase promoter. I already tried ATCC, entrez, google and I
>>didn't find neither the map nor the sequence. All I found was a
>>reference to a paper from 1988.
> ATCC sells the vector (cat. # 37584) - maybe write them asking for
> a deposited sequence. Alternatively, ask the depositor:
> Steven Nordeen, Steve.Nordeen from UCDenver.edu - maybe he still
> has the sequence somewhere on floppy disk :-)
My recollection is that Nordeen constructed his plasmids as derivatives
of pSVL (de Wet et al., MCB 7:725, 1987). The details of construction are
described in Nordeen's Biotechniques paper (6:454, 1988).
I have reconstructed a sequence for pSVL - which I would be happy to
email you. Using it, and Nordeen's construction details, it should be
relatively simple to reconstruct the sequence of pT81luc.
Email: echo 36434455860060025978157675027927670979097959886449930P | dc
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