CHX chase: what is a good control?

AS via methods%40net.bio.net (by Antonio.Sarikas from gmail.com)
Fri Feb 1 15:27:45 EST 2008


I am looking for a cytoplasmatic protein with a halflife of approx 2
hrs to use as a control in a CHX chase. (not as a loading control). My
concern is that CHX can kill the cells thereby releasing cytoplasmic
proteins while the "cell skeleton" with actin remains, resulting in a
decay of your protein of interest, that is actually not real.


Thank you for your opinion!


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