CHX chase: what is a good control?

AS via (by Antonio.Sarikas from
Fri Feb 1 15:27:45 EST 2008

I am looking for a cytoplasmatic protein with a halflife of approx 2
hrs to use as a control in a CHX chase. (not as a loading control). My
concern is that CHX can kill the cells thereby releasing cytoplasmic
proteins while the "cell skeleton" with actin remains, resulting in a
decay of your protein of interest, that is actually not real.

Thank you for your opinion!

More information about the Methods mailing list