CHX chase: what is a good control?

Aawara Chowdhury via methods%40net.bio.net (by aawara from pontiff-playground.org)
Sat Feb 2 21:44:46 EST 2008


In <ac714651-d6a8-4b73-a82e-f9d273229634 from i29g2000prf.googlegroups.com>,
 AS <Antonio.Sarikas from gmail.com> wrote:

>
> I am looking for a cytoplasmatic protein with a halflife of approx 2
> hrs to use as a control in a CHX chase. (not as a loading control). My
> concern is that CHX can kill the cells thereby releasing cytoplasmic
> proteins while the "cell skeleton" with actin remains, resulting in a
> decay of your protein of interest, that is actually not real.
>

How about d2EGFP?  It is a derivative of EGFP with a PEST sequence that
reduces its half-life to about 2 hours.  Clontech used to sell it.

AC
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