How to prepare 1M H2O2 using 30% hydrogen peroxide?

Virash Gupta via methods%40net.bio.net (by virashkgupta from gmail.com)
Fri Feb 8 08:15:31 EST 2008


30 % H2O2 is 8.84 M solution. Dilute 8.84 times. The problem is solved.
all the best



On Feb 7, 2008 10:34 PM, <methods-request from oat.bio.indiana.edu> wrote:

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>   1. R: buffer preparation (Prof. Piero Sestili)
>   2. Dulbecco`s PBS vs PBS pH7.4 (AS)
>   3. Re: How to prepare 1M H2O2 using 30% hydrogen peroxide?
>      (Pow Joshi)
>   4. RE:Induction of apoptosis (Ozan Aygun)
>   5. Re: Dulbecco`s PBS vs PBS pH7.4 (DK)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 6 Feb 2008 16:02:26 +0100
> From: "Prof. Piero Sestili" <piero.sestili from uniurb.it>
> Subject: R: buffer preparation
> To: <bertrutten from gmail.com>, <methods from magpie.bio.indiana.edu>
> Message-ID: <002501c868d1$4d9d7f00$7d0ca8c0 from iram.it>
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> First prepare a 2 M solution by diluting 2.26 ml of your stock to 10 ml,
> then make serial 1:10 dilutions down to 2 mM
> or
> prepare a 200 mM solution  first diluting 226 ul of your stock to 10 ml
> and then another 1:100 dilution to 2 mM.  As you prefer....
>
>
> Piero
>
>
> Prof. Piero Sestili
> Istituto di Farmacologia e Farmacognosia e
> Centro di Ricerca sull'Attività Motoria
> Università degli Studi di Urbino "Carlo Bo"
> Via "I Maggetti" 26
> 61029 URBINO (PU)
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>
> -----Messaggio originale-----
> Da: methods-bounces from oat.bio.indiana.edu
> [mailto:methods-bounces from oat.bio.indiana.edu] Per conto di
> bertrutten from gmail.com
> Inviato: mercoledì 6 febbraio 2008 12.11
> A: methods from magpie.bio.indiana.edu
> Oggetto: buffer preparation
>
> Hello,
>
> How do i work this out?
> :
>
> I need 2mM H2O2 from a stock solution H2O2 of 30%, density 1.11 gr/ml,
> M.W. 34
>
>
> anybody?
> thanks upfront
>
> b
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>
> ------------------------------
>
> Message: 2
> Date: Wed, 6 Feb 2008 11:39:05 -0800 (PST)
> From: AS <Antonio.Sarikas from gmail.com>
> Subject: Dulbecco`s PBS vs PBS pH7.4
> To: methods from net.bio.net
> Message-ID:
>        <8dbed6dc-131a-4266-bfb8-e900ef4c575c from e23g2000prf.googlegroups.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> What is the difference between Dulbecco`s PBS vs PBS pH7.4?
>
> Does it make a difference if I just use it to wash cells (293, MEFs
> etc) before trypsination or lysation?
>
> Thanks!
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 6 Feb 2008 13:09:45 -0500
> From: "Pow Joshi" <pow.joshi from gmail.com>
> Subject: Re: How to prepare 1M H2O2 using 30% hydrogen peroxide?
> To: DK <dk from no.email.thankstospam.net>
> Cc: methods from magpie.bio.indiana.edu
> Message-ID:
>        <710764ea0802061009j76567393l72fa9680a3196de2 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> On 05/02/2008, DK <dk from no.email.thankstospam.net> wrote:
> >
> > In article <
> > 4f8737e1-bbe4-4542-8c1e-e98c7cddab5a from e6g2000prf.googlegroups.com>, WS <
> > novalidaddress from nurfuerspam.de> wrote:
> > >Dear Lao,
> > >
> > >that's the real issue. All your assumptions may be true.
> > >
> > >IMHO is is *absolutely* necessary to specify m/v (mass/volume), m/m or
> > >v/v. It even makes a difference if you (in this case rather
> > >hypothetically as solid H2O2 does not exist [at least for a long
> > >time]) mix 300 ml of H2O2 and 700ml of water, put 300 ml H2O2 in a
> > >beaker and add water up to 1liter or vice versa.
> >
> > Industrial % concentrations tend to be m/m. This is clearly
> > the case, for example, with concentrated HCl (~ 37% ~ 12M)
> > or concentrated H3PO4 (85% = 15.2M).
> >
> > MSDS for 30% H2O2 says "balance water", suggesting
> > that indeed we are talking about 300 g + 700 g water.
> > In which case precise molarity calculattion become
> > troublesome even knowing density of 100% H2O2
> > because water solutions can change volume
> > appreciably (e.g. 500 ml ethanol + 500 ml H2O
> > will result in less than 1000 ml solution).
> >
> > Not that any of that matters in the experiment in
> > question! Assuming no volume change and m/m
> > for 30% and density off 199% of 1.4 g/ml from
> > Wikipedia, we get 22-28% error depending on the
> > direction. With the "1 M" being clearly an arbitrary
> > round number, I wouln't worry about potential
> > 25% error as long as the way the solution is prepared
> > is clearly stated.
>
>
>
> yes, that is true.... H2O2 would be w/w.... the MSDS generally does'nt
> give
> the information, however, calling the company would give. Frankly, I
> did'nt
> know that this is true for all industrial preparation..... Thank you Dima.
>
> Pow
>
> DK
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>
>
> ------------------------------
>
> Message: 4
> Date: Wed, 6 Feb 2008 13:45:51 -0800 (PST)
> From: Ozan Aygun <metugenetics from yahoo.com>
> Subject: RE:Induction of apoptosis
> To: methods from magpie.bio.indiana.edu
> Message-ID: <16475.93161.qm from web90402.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi,
>
> You may want to give try alpha-amanitin, which is a
> highly specific inhibitor of RNA polymerase II. It is
> known that exposure to this reagent causes apoptosis
> in human cells.
>
> Good luck,
>
> Ozan
>
>
>
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> ------------------------------
>
> Message: 5
> Date: Wed, 06 Feb 2008 22:25:02 GMT
> From: dk from no.email.thankstospam.net (DK)
> Subject: Re: Dulbecco`s PBS vs PBS pH7.4
> To: methods from net.bio.net
> Message-ID: <1tqqj.50$iB4.41 from newsfe07.lga>
>
> In article <
> 8dbed6dc-131a-4266-bfb8-e900ef4c575c from e23g2000prf.googlegroups.com>, AS <
> Antonio.Sarikas from gmail.com> wrote:
> >What is the difference between Dulbecco`s PBS vs PBS pH7.4?
>
> Probably none. Gibco used to specify its Dulbecco's PBS
> PH as 7.0-7.5. "PBS" is a mess. It can refer to a number of
> similar solutions, each with and without Ca2+ and/or Mg2+.
>
> >Does it make a difference if I just use it to wash cells (293, MEFs
> >etc) before trypsination or lysation?
>
> In the range in question, pH won't make a difference but for
> trypsinization there might be a diffrence between PBS
> containing divalents or not. Don't think it will be significant
> if you use trypsin-EDTA.
>
> DK
>
>
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> End of Methods Digest, Vol 33, Issue 6
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-- 
Dr V K Gupta
Sr Microbiologist (Molecular Biology)
Insect Molecular Biology Lab
Department of Entomology
Punjab Agricultural University
Ludhiana (Pb)-141004- India
M: 09815963210


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