Enterohaemorrhagic Escherichia coli

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Sat Feb 9 17:25:42 EST 2008

Dear Pooja,

that mostly depends on you local governmental regulations. In general,
the best thing to do for you is to ask the people in charge for
biosafety issues at your facility.

Here in Germany, it would be at least biosafety lavel 2 (L2) for work
with whole pathogenic bacteria and genetic engineering safety lavel 2,
too (S2) if you work with single genes from such an organism.

If you plan to investigate with factors that influence virulence, the
safety standards to follow will be higher than if you just want to do
genotyping by sequencing some probably not dangerous parts of their
chromosomes in order to characterize isolates from the clinic.

Another issue might be that you don't want to contaminate any standard
lab cultures of E. coli with this probably quite nasty bug. Not to
imagine what will happen if people start to grow their DNA preps in
hemorragic E. coli strains, not being aware of what is lurking inside
their flasks. Getting in contact with large amounts of 'your' strain
probably will cause serious health issues to everyone affected. And in
the end to you: When they find out where the bug came from, they will
chase you to the South Pole or to Mars and Venus :-). So you probably
want to work in well separated labs and also have all necessary
precautions for efficient decontamination of all used labware and
disposals containing your bacteria.

Have fun,


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