Enterohaemorrhagic Escherichia coli

Sudheendra Rao N R via methods%40net.bio.net (by sudhee26 from gmail.com)
Sun Feb 10 00:06:50 EST 2008


Hi Pooja,

Under DBT guidelines all Entero Pathogenic strains of E Coli come under Risk
Category II

http://dbtbiosafety.nic.in/act/_Annex-4.htm

Try mailing them or contacting a local wing of that.

Sudheendra.


On Feb 10, 2008 10:36 AM, Sudheendra Rao N R <sudhee26 from gmail.com> wrote:

>
>
>   On Feb 10, 2008 3:55 AM, WS <novalidaddress from nurfuerspam.de> wrote:
>
> > Dear Pooja,
> >
> > that mostly depends on you local governmental regulations. In general,
> > the best thing to do for you is to ask the people in charge for
> > biosafety issues at your facility.
> >
> > Here in Germany, it would be at least biosafety lavel 2 (L2) for work
> > with whole pathogenic bacteria and genetic engineering safety lavel 2,
> > too (S2) if you work with single genes from such an organism.
> >
> > If you plan to investigate with factors that influence virulence, the
> > safety standards to follow will be higher than if you just want to do
> > genotyping by sequencing some probably not dangerous parts of their
> > chromosomes in order to characterize isolates from the clinic.
> >
> > Another issue might be that you don't want to contaminate any standard
> > lab cultures of E. coli with this probably quite nasty bug. Not to
> > imagine what will happen if people start to grow their DNA preps in
> > hemorragic E. coli strains, not being aware of what is lurking inside
> > their flasks. Getting in contact with large amounts of 'your' strain
> > probably will cause serious health issues to everyone affected. And in
> > the end to you: When they find out where the bug came from, they will
> > chase you to the South Pole or to Mars and Venus :-). So you probably
> > want to work in well separated labs and also have all necessary
> > precautions for efficient decontamination of all used labware and
> > disposals containing your bacteria.
> >
> > Have fun,
> >
> > Wo
> >  _______________________________________________
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> > Methods from net.bio.net
> > http://www.bio.net/biomail/listinfo/methods
> >
>
>
>
> --
> Think before agree
> Think before you nod
> but STOP thinking
> and You Are God




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Think before agree
Think before you nod
but STOP thinking
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