site directed mutagensis

doaa zineldeen via methods%40net.bio.net (by zineldeen from gmail.com)
Sat Feb 9 23:25:44 EST 2008


hello
I just want if anyone have a protocol for pcr mediated site directed
mutagensis without using stratagene kit
i have a template vector containing the insert and i want to mutate k81 into
A i designed 2 primers flanking the mutation site and i want can i perform 2
pcr steps and a third mix with normal polymerase i am using phusion
can i proceed to get the new product containing the mutated sites
If someone can help i am really grateful
Doaa


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