site directed mutagensis

[doaa zineldeen]

hello
I just want if anyone have a protocol for pcr mediated site directed
mutagensis without using stratagene kit
i have a template vector containing the insert and i want to mutate k81 into
A i designed 2 primers flanking the mutation site and i want can i perform 2
pcr steps and a third mix with normal polymerase i am using phusion
can i proceed to get the new product containing the mutated sites
If someone can help i am really grateful
Doaa