Calcium binding protein assay

[Jose Olucha]

First time I post something in the methods mail list (very exciting)

Anywhoo...

I am working with a CIB1 (calcium binding protein involved in cell
development, migration and differentiation) ortholog(F30A10.1) in C.
elegans. I was looking for any quick and easy calcium binding assays that I
could use as a possible activity assay.


Also question number 2:

would anyone know of a reason why WB would not work using nitrocellulose as
blotting membrane, as opposed to PVDF? Blots of this protein work with PVDF
membrane but do not work with nitrocellulose. Any ideas why?