Can you heat RPMI 1640?
Dr Engelbert Buxbaum
(by engelbert_buxbaum from hotmail.com)
Mon Feb 18 11:12:25 EST 2008
Am 07.02.2008, 08:39 Uhr, schrieb Kamalian, Laleh
<L.Kamalian from liverpool.ac.uk>:
> I have found the concentration of the components and every thing else.
> The only problem is how to dissolve methylcellulose in the media. I
> found out that I have to add warm (60 ' C) liquid to the powder and let
> it cool down while stirring. But can I heat RPMI 1640 media to 60"? I
> couldn't find the answer on the net.
I would produce a 10 or 20 x stock solution of Methylcellulose in water
and autoclave to get it sterile. Then use 10 x RPMI (commercially
available or prepared from powder) to make the final medium.
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