Inducer of apoptosis in animal

WANG XF via methods%40net.bio.net (by wxfhome from gmail.com)
Wed Feb 20 20:25:29 EST 2008


 Thanks a lot you all!

Sorry!  I did not express clearly in last post. What I mean is to induce
apoptosis in some tissue of the mouse, but not the whole animal.

Thanks again for your help!

LW


Today's Topics:
>
>   1. Inducer of apoptosis in animal (wxfhome from gmail.com)
>   2. Re: Inducer of apoptosis in animal (Kyle Legate)
>   3. Re: Inducer of apoptosis in animal (Pow Joshi)
>   4. Re: Inducer of apoptosis in animal (WS)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 19 Feb 2008 01:41:23 -0800 (PST)
> From: wxfhome from gmail.com
> Subject: Inducer of apoptosis in animal
> To: methods from net.bio.net
> Message-ID:
>        <47f74ecd-2335-4171-8ee6-963c78c4789d from e10g2000prf.googlegroups.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello!
>
> I want to test a method which can detect apoptosis in mice, and I do
> not know how to induce apoptosis in mice. Could anyone give some
> suggestions about the inducer and protocol? I see H2O2 can induce
> apoptosis in cell culture.
>
> Thank you all.
>
> LW
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 19 Feb 2008 19:19:25 +0100
> From: Kyle Legate <legatek from hotmail.com>
> Subject: Re: Inducer of apoptosis in animal
> To: methods from net.bio.net
> Message-ID: <620kt8F20kj81U1 from mid.individual.net>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> wxfhome from gmail.com wrote:
> > Hello!
> >
> > I want to test a method which can detect apoptosis in mice, and I do
> > not know how to induce apoptosis in mice. Could anyone give some
> > suggestions about the inducer and protocol? I see H2O2 can induce
> > apoptosis in cell culture.
> >
> I'm not sure that inducing apoptosis in a whole mouse would pass ethics
> boards. I know they wouldn't see it as necessary. Select a tissue which
> is known to have a significant amount of natural apoptosis, like the
> skin during hair follicle catagen (around 8 weeks after birth) or the
> mammary gland sometime after weaning, during involution, and test your
> method on those samples.
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 19 Feb 2008 13:47:59 -0500
> From: "Pow Joshi" <pow.joshi from gmail.com>
> Subject: Re: Inducer of apoptosis in animal
> To: "wxfhome from gmail.com" <wxfhome from gmail.com>
> Cc: methods from magpie.bio.indiana.edu
> Message-ID:
>        <710764ea0802191047j556899c5h93fe88a5c75804bf from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> On 19/02/2008, wxfhome from gmail.com <wxfhome from gmail.com> wrote:
> >
> > Hello!
> >
> > I want to test a method which can detect apoptosis in mice, and I do
> > not know how to induce apoptosis in mice. Could anyone give some
> > suggestions about the inducer and protocol? I see H2O2 can induce
> > apoptosis in cell culture.
>
>
>
> hello, you mean you want to  induce apoptosis in vivo?  Does it have a
> specific region of an organ system? Because, an apoptosed mouse, I'd
> imagine  is a very dead mouse.
>
> Pow
>
> Thank you all.
> >
> > LW
> > _______________________________________________
> > Methods mailing list
> > Methods from net.bio.net
> > http://www.bio.net/biomail/listinfo/methods
> >
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 19 Feb 2008 14:07:30 -0800 (PST)
> From: WS <novalidaddress from nurfuerspam.de>
> Subject: Re: Inducer of apoptosis in animal
> To: methods from net.bio.net
> Message-ID:
>        <3a5cd921-ee77-48f8-ae19-5014ccb3ac7f from p73g2000hsd.googlegroups.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
>
> > Because, an apoptosed mouse, I'd
> > imagine  is a very dead mouse.
> >
> > Pow
>
> Yes, very dead indeed. In German, this is called "mausetot" (as dead
> as a mouse...)
>
> What about taking out the organs of interest, perfusing them like in
> the Langendorf Heart Model and include apoptosis inducers in the
> buffer? As lethal to mice as whole animal apoptosis, but a bit more
> human...
>
> Best,
>
> Wo
>
>


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