Inducer of apoptosis in animal

Sudheendra Rao N R via methods%40net.bio.net (by sudhee26 from gmail.com)
Wed Feb 20 23:18:27 EST 2008


How about using Lipofectamine?? stereotaxically injecting wherever you
want??
in culture it surely causes apoptosis.
i guess a cheaper alternative would be better anyway :)

Sudheendra.

On Thu, Feb 21, 2008 at 6:55 AM, WANG XF <wxfhome from gmail.com> wrote:

>  Thanks a lot you all!
>
> Sorry!  I did not express clearly in last post. What I mean is to induce
> apoptosis in some tissue of the mouse, but not the whole animal.
>
> Thanks again for your help!
>
> LW
>
>
> Today's Topics:
> >
> >   1. Inducer of apoptosis in animal (wxfhome from gmail.com)
> >   2. Re: Inducer of apoptosis in animal (Kyle Legate)
> >   3. Re: Inducer of apoptosis in animal (Pow Joshi)
> >   4. Re: Inducer of apoptosis in animal (WS)
> >
> >
> > ----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Tue, 19 Feb 2008 01:41:23 -0800 (PST)
> > From: wxfhome from gmail.com
> > Subject: Inducer of apoptosis in animal
> > To: methods from net.bio.net
> > Message-ID:
> >        <
> 47f74ecd-2335-4171-8ee6-963c78c4789d from e10g2000prf.googlegroups.com>
> > Content-Type: text/plain; charset=ISO-8859-1
> >
> > Hello!
> >
> > I want to test a method which can detect apoptosis in mice, and I do
> > not know how to induce apoptosis in mice. Could anyone give some
> > suggestions about the inducer and protocol? I see H2O2 can induce
> > apoptosis in cell culture.
> >
> > Thank you all.
> >
> > LW
> >
> >
> > ------------------------------
> >
> > Message: 2
> > Date: Tue, 19 Feb 2008 19:19:25 +0100
> > From: Kyle Legate <legatek from hotmail.com>
> > Subject: Re: Inducer of apoptosis in animal
> > To: methods from net.bio.net
> > Message-ID: <620kt8F20kj81U1 from mid.individual.net>
> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> >
> > wxfhome from gmail.com wrote:
> > > Hello!
> > >
> > > I want to test a method which can detect apoptosis in mice, and I do
> > > not know how to induce apoptosis in mice. Could anyone give some
> > > suggestions about the inducer and protocol? I see H2O2 can induce
> > > apoptosis in cell culture.
> > >
> > I'm not sure that inducing apoptosis in a whole mouse would pass ethics
> > boards. I know they wouldn't see it as necessary. Select a tissue which
> > is known to have a significant amount of natural apoptosis, like the
> > skin during hair follicle catagen (around 8 weeks after birth) or the
> > mammary gland sometime after weaning, during involution, and test your
> > method on those samples.
> >
> >
> > ------------------------------
> >
> > Message: 3
> > Date: Tue, 19 Feb 2008 13:47:59 -0500
> > From: "Pow Joshi" <pow.joshi from gmail.com>
> > Subject: Re: Inducer of apoptosis in animal
> > To: "wxfhome from gmail.com" <wxfhome from gmail.com>
> > Cc: methods from magpie.bio.indiana.edu
> > Message-ID:
> >        <710764ea0802191047j556899c5h93fe88a5c75804bf from mail.gmail.com>
> > Content-Type: text/plain; charset=ISO-8859-1
> >
> > On 19/02/2008, wxfhome from gmail.com <wxfhome from gmail.com> wrote:
> > >
> > > Hello!
> > >
> > > I want to test a method which can detect apoptosis in mice, and I do
> > > not know how to induce apoptosis in mice. Could anyone give some
> > > suggestions about the inducer and protocol? I see H2O2 can induce
> > > apoptosis in cell culture.
> >
> >
> >
> > hello, you mean you want to  induce apoptosis in vivo?  Does it have a
> > specific region of an organ system? Because, an apoptosed mouse, I'd
> > imagine  is a very dead mouse.
> >
> > Pow
> >
> > Thank you all.
> > >
> > > LW
> > > _______________________________________________
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> > > Methods from net.bio.net
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> > >
> >
> >
> > ------------------------------
> >
> > Message: 4
> > Date: Tue, 19 Feb 2008 14:07:30 -0800 (PST)
> > From: WS <novalidaddress from nurfuerspam.de>
> > Subject: Re: Inducer of apoptosis in animal
> > To: methods from net.bio.net
> > Message-ID:
> >        <
> 3a5cd921-ee77-48f8-ae19-5014ccb3ac7f from p73g2000hsd.googlegroups.com>
> > Content-Type: text/plain; charset=ISO-8859-1
> >
> >
> > > Because, an apoptosed mouse, I'd
> > > imagine  is a very dead mouse.
> > >
> > > Pow
> >
> > Yes, very dead indeed. In German, this is called "mausetot" (as dead
> > as a mouse...)
> >
> > What about taking out the organs of interest, perfusing them like in
> > the Langendorf Heart Model and include apoptosis inducers in the
> > buffer? As lethal to mice as whole animal apoptosis, but a bit more
> > human...
> >
> > Best,
> >
> > Wo
>  >
> >
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