pcr prob

harold b via methods%40net.bio.net (by theoharis from gmail.com)
Sat Feb 23 11:20:09 EST 2008


Hi, my negative control (no template with both primers and mastermix)
consistently shows a stronger single band whereas my amplicons show a
leading similar but weaker band followed by a fading smear reminiscent
of non-amplified DNA. In each case the product with DNA is less
intense. Can someone make sense of this?


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