Methods Digest, Vol 33, Issue 25 pcr prob

rijuta kotenkar via methods%40net.bio.net (by rijutak from googlemail.com)
Mon Feb 25 02:19:20 EST 2008


Hi!
Did you check if your primers form Primer-dimer?? Or check if your primer
has been contaminated by the template.

Rijuta

On Sun, Feb 24, 2008 at 6:03 PM, <methods-request from oat.bio.indiana.edu>
wrote:

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>   1. pcr prob (harold b)
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> Date: Sat, 23 Feb 2008 08:20:09 -0800 (PST)
> From: harold  b <theoharis from gmail.com>
> Subject: pcr prob
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> Hi, my negative control (no template with both primers and mastermix)
> consistently shows a stronger single band whereas my amplicons show a
> leading similar but weaker band followed by a fading smear reminiscent
> of non-amplified DNA. In each case the product with DNA is less
> intense. Can someone make sense of this?
>
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