pcr prob

[Aawara Chowdhury]

In <1203951168.24775.0 from proxy01.news.clara.net>,
 ChenHA <hzhen from freeuk.com> wrote:

> Certainly from the evidence more intelligent than you.  Which bit of the 
> "unwarranted assumption on primer concentration and length" don't you 
> understand?  What is the length of primers used?  You don't know (mine 
> are usually more than 30 but less than 60, very occasionally 80 or 
> more).  Do they fold back?  Possibly, or perhaps they form loops, or 
> whatever (and very probably, these have no relevance to what happened in 
> hot reactions compare to what might happen in a room temperature gel). 
> Do they form primer dimer in the reaction, maybe, maybe not, but from my 
> own experience they usually aren't primer dimers (and they may indeed 
> form dimer in gel, but that may have no relevance to what happened in 
> the reaction).  You are arguing from no experience, read a few things 
> then spouting off about things you have no experience of.
> Idiot.

Highly unlikely you have primers that dimerize so stably that they bind
ethidium.  And if they did, they'd be very poor as primers for PCR.

Nonethess, your name-calling doesn't remove the fact that you continue
to claim, in the face of plenty of publications demonstrating the latter,
that single-stranded primers bind EtBr.

Wonder what your reaction is like to critiques of manuscripts, if you've
submitted any.

AC
-- 
Email: echo 36434455860060025978157675027927670979097959886449930P | dc