(by hzhen from freeuk.com)
Mon Feb 25 10:21:30 EST 2008
Aawara Chowdhury wrote:
> In <1203951168.24775.0 from proxy01.news.clara.net>,
> ChenHA <hzhen from freeuk.com> wrote:
>> Certainly from the evidence more intelligent than you. Which bit of the
>> "unwarranted assumption on primer concentration and length" don't you
>> understand? What is the length of primers used? You don't know (mine
>> are usually more than 30 but less than 60, very occasionally 80 or
>> more). Do they fold back? Possibly, or perhaps they form loops, or
>> whatever (and very probably, these have no relevance to what happened in
>> hot reactions compare to what might happen in a room temperature gel).
>> Do they form primer dimer in the reaction, maybe, maybe not, but from my
>> own experience they usually aren't primer dimers (and they may indeed
>> form dimer in gel, but that may have no relevance to what happened in
>> the reaction). You are arguing from no experience, read a few things
>> then spouting off about things you have no experience of.
> Highly unlikely you have primers that dimerize so stably that they bind
> ethidium. And if they did, they'd be very poor as primers for PCR.
> Nonethess, your name-calling doesn't remove the fact that you continue
> to claim, in the face of plenty of publications demonstrating the latter,
> that single-stranded primers bind EtBr.
> Wonder what your reaction is like to critiques of manuscripts, if you've
> submitted any.
Jesus, you are dim.
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