pcr prob

Aawara Chowdhury via methods%40net.bio.net (by aawara from pontiff-playground.org)
Tue Feb 26 02:12:54 EST 2008


In <nYKwj.530$Td5.229 from newsfe07.lga>,
 DK <dk from no.email.thankstospam.net> wrote:

> Interesting. I never thought about it. Will do this control next 
> time I PCR something. I suppose not adding template will be 
> a good way to make amplification inefficient :-)

There's an old paper in Nucleic Acids Research that describes the
formation of primer-dimers and a method to eliminate their formation.

The elimination of primer-dimer accumulation in PCR, Nuc. Acids Res.
25: 3235-3241 (1995).

AC
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