Debate

Aawara Chowdhury via methods%40net.bio.net (by aawara from pontiff-playground.org)
Fri Feb 29 07:47:05 EST 2008


In <sBSxj.3$DO4.0 from newsfe02.lga>,
 DK <dk from no.email.thankstospam.net> wrote:

>>There is a relatively new alternative to GFP for fluorescent tagging of
>>proteins in live cells in the shape of the FlAsH/ReAsH system:
>>
>>http://www.invitrogen.com/content.cfm?pageid=11922
>>
>>Basically, you put a magic tag on your protein, 
>
> The magic tag with two exposed Cys is a subject to many
> crosslinking artifacts. Probably not a problem for most 
> cytoplasmic proteins (reducing environment) but must
> be a huge problem for anything that goes through secretion
> pathway. 

Any thoughts on the SNAP tag?
http://www.covalys.com/products/snap-cell-labeling-in-cells.html

I've been considering purchasing it.  

AC
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