pcr prob

Aawara Chowdhury via methods%40net.bio.net (by aawara from pontiff-playground.org)
Mon Feb 25 08:16:04 EST 2008


In <1203944654.12385.0 from proxy02.news.clara.net>,
 ChenHA <hzhen from freeuk.com> wrote:

> Are you in the habit of teaching your grandma to suck eggs?  Perhaps I 
> should have added when I said that I have seen it often before, that I 
> have also checked with running the primers by itself on the gel, and saw 
> a bright glowing band?  

And I never have, even back in the day when cycling was done by moving
reactions from block to block, and primers were used at 200 micromolar.  
And based on how ethidium works, your primers must either snap-back upon 
themselves or anneal to each other; either way an odd primer design.

AC

P.S. You don't have to be so rude, just because someone points out
an incongruence in your "observation".

-- 
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