About signal peptide
Trond Erik Vee Aune
(by trondaun from nt.ntnu.no)
Wed Jan 2 02:36:21 EST 2008
WANG XF wrote:
> Hello, everyone!
> I have a question about protein secretion. I want to develop a construct as
> *promoter----protein 1--protease site---signal peptide---protein 2*
> If the two proteins was expressed and cleaved at the protease site, protein
> 2 will be secreted into the supernatant?
I assume the signal peptide is for Sec-mediated translocation to the E.
coli periplasm. E. coli does not efficiently secrete proteins, but
over-expression with translocation to periplasm may lead to lysis,
addition of chemicals that disrupt the outer membrane may lead to
Mergulhão, F. J. M., D. K. Summers and G. A. Monteiro (2005).
"Recombinant protein secretion in Escherichia coli." Biotechnol. Adv.
Mergulhão, F. J. M., M. A. Taipa, J. M. S. Cabral and G. A. Monteiro
(2004). "Evaluation of bottlenecks in proinsulin secretion by
Escherichia coli." J. Biotechnol. 109(1-2): 31-43.
Francetic, O., D. Belin, C. Badaut and A. P. Pugsley (2000). "Expression
of the endogenous type II secretion pathway in Escherichia coli leads to
chitinase secretion." EMBO. J. 19(24): 6697-6703.
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